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May 01, 2012
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Total reflexão interna de fluorescência microscopia (TIRF) é uma abordagem poderosa para observar as estruturas perto da superfície da célula em alto contraste e resolução temporal. Demonstramos como TIRF pode ser empregue para estudar a dinâmica de proteína no córtex de parede celular fechados células bacterianas e fúngicas.
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Cite this Article
Spira, F., Dominguez-Escobar, J., Müller, N., Wedlich-Söldner, R. Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy. J. Vis. Exp. (63), e3982, doi:10.3791/3982 (2012).
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