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JoVE Journal Bioengineering
Visualisering av Cortex Organisation och Dynamics i mikroorganismer, med total inre Mikroskopi fluorescens
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Visualisering av Cortex Organisation och Dynamics i mikroorganismer, med total inre Mikroskopi fluorescens

Article DOI: 10.3791/3982-v • 14:15 min • May 1st, 2012
May 1st, 2012 •
1, 1, 1,2, 1
1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München

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Fluorescens vid total internreflexion (TIRF) mikroskopi är en kraftfull metod för att observera strukturer nära cellens yta vid hög kontrast och temporal upplösning. Vi visa hur TIRF kan användas för att studera protein-dynamiken vid cortex av cellväggen slutna bakterie-och svampceller.

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Bioteknik TIRF bildhantering jäst bakterier mikroskopi cell cortex
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