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JoVE Journal Bioengineering
Visualisatie van Cortex Organisatie en Dynamiek in Micro-organismen, met behulp van Total Internal Reflection Fluorescence Microscopy
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Visualisatie van Cortex Organisatie en Dynamiek in Micro-organismen, met behulp van Total Internal Reflection Fluorescence Microscopy

Article DOI: 10.3791/3982-v • 14:15 min • May 1st, 2012
May 1st, 2012 •
1, 1, 1,2, 1
1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München

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Total Internal Reflection Fluorescence (TIRF) microscopie is een krachtige aanpak van de structuren te observeren in de buurt van het celoppervlak bij hoge contrast en temporele resolutie. We laten zien hoe TIRF kan worden gebruikt om eiwitten dynamiek te studeren aan de cortex van de celwand-ingesloten bacteriën en schimmels cellen.

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Bioengineering TIRF imaging gisten bacteriën microscopie cel cortex
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