Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

聚合酶链反应:基本协议加上故障排除和优化策略
 
Click here for the English version

聚合酶链反应:基本协议加上故障排除和优化策略

Article DOI: 10.3791/3998-v 09:00 min May 22nd, 2012
May 22nd, 2012

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

PCR技术已经成为了许多分子生物学实验室常用技术。这里提供几个常规PCR协议是一个快速指导。因为每一个反应是一个独特的试验,以产生不同产品所需的最佳条件。了解反应中的变量,将大大提高故障排除效率,从而增加了机会,以获得所需的结果。

Tags

基本协议,63期,PCR技术,优化,设计引物,熔融温度,T
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter