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Journal / Neuroscience / Generering af en immortaliseret murin Brain…
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Neuroscience

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JoVE Journal Neuroscience
Generering af en immortaliseret murin Brain mikrovaskulær endotel cellelinie som en
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Generering af en immortaliseret murin Brain mikrovaskulær endotel cellelinie som en

Article DOI: 10.3791/4022-v • 09:37 min • August 29th, 2012
August 29th, 2012 •
1, 1, 1
1Klinik und Poliklinik für Anästhesiologie, University of Wurzburg

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Denne metode beskriver hvordan du isolerer og udødeliggøre mikrovaskulære endotelceller fra musehjerne. Vi beskriver en trin-for-trin-protokollen begyndende fra homogenisering af hjernevævet, fordøjelse trin, podning og immortalisering af cellerne. Normalt vil det tage omkring fem uger for at opnå en homogen, immortaliseret mikrovaskulære endotel-cellelinie.

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Immunology Neuroscience blod-hjerne-barrieren, hjerne mikrovaskulære endotelceller immortalisering cEND
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