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核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站
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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Please note that all translations are automatically generated. Click here for the English version.

核糖体束缚新生多肽的分离<em>在体外</em>确定沿mRNA的翻译暂停网站

English

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16,197 Views

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10:15 min

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July 06, 2012

DOI:

10.3791/4026-v

DOI:
10.3791/4026-v

•

10:15 min
July 06, 2012

•

2 Views
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1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Summary

Automatically generated

描述的一种技术，以确定mRNA的翻译暂停网站。本程序是基于对新生多肽在体外培养的靶mRNA的翻译，其次是使用变性凝胶电泳新生链的大小分析核糖体积累的隔离。

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RibosomePolypeptidesTranslational Pause SitesMRNACodon UsageSynonymous CodonsNon-uniform ElongationMRNA Secondary StructureRibosome MovementTranslational Elongation RatesCodon BiasTRNAsRare CodonsPause SitesProtein ExpressionMRNA StabilityProtein Folding

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Cite this Article

Jha, S. S., Komar, A. A. Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA. J. Vis. Exp. (65), e4026, doi:10.3791/4026 (2012).

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