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Isolering av ribosome Innbundne begynnende polypeptider
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Isolering av ribosome Innbundne begynnende polypeptider

Article DOI: 10.3791/4026 • 10:15 min
July 6th, 2012 •
1, 1
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University

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July 6th, 2012

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En teknikk for å identifisere translasjonelle pause områder på mRNA blir beskrevet. Denne prosedyren er basert på isolasjon av begynnende polypeptider samler på ribosomer under in vitro oversettelse av et mål mRNA, etterfulgt av størrelsen analyse av de begynnende kjedene bruker en denaturering gel elektroforese.

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