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JoVE Journal Neuroscience
Produksjon av Lentiviral vektorer for Transducing Celler fra Central Nervous System
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Produksjon av Lentiviral vektorer for Transducing Celler fra Central Nervous System

Article DOI: 10.3791/4031-v • 08:46 min • May 24th, 2012
May 24th, 2012 •
1, 1, 1, 1
1Department of Neurology and Hope Center for Neurological Disorders, Washington University School of Medicine

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I denne protokollen beskriver vi produksjon, rensing og titrering av lentiviral vektorer. Vi tilbyr et eksempel på lentiviral vektor-mediert genet levering i primære dyrkede nerveceller og astrocytes. Våre metoder kan også gjelde for andre celletyper

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Neuroscience cellekultur transduksjon lentiviral vektor nervecellen astrocyte promoter CNS genetikk
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