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En bekvem og General Expression Platform for produktion af secernerede proteiner fra humane celler
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En bekvem og General Expression Platform for produktion af secernerede proteiner fra humane celler

Article DOI: 10.3791/4041-v • 07:10 min • July 31st, 2012
July 31st, 2012 •
*1, *1, *1, 1
1Department of Laboratory Medicine and Pathobiology, University of Toronto
* These authors contributed equally

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I den post-humane genom-æraen, er tilgængeligheden af ​​rekombinante proteiner i native konformationer afgørende for strukturel, funktionel og terapeutisk forskning og udvikling. Her beskriver vi en test-og store protein-ekspressionssystem i humane embryoniske nyre 293T-celler, som kan anvendes til fremstilling af en række af rekombinante proteiner.

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Genetik Medicine Molecular Biology Human proteinekspression HEK 293T glycoproteiner cytokiner cellulære celleoverfladereceptorer ekstracellulære proteiner restriktionsenzymer faktorer virale proteiner mammal ekspression protokol high-throughput
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