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Journal / Neuroscience / 阈上的神经活动的单细胞和单穗的分辨率的光学的方法记录…
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阈上的神经活动的单细胞和单穗的分辨率的光学的方法记录
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阈上的神经活动的单细胞和单穗的分辨率的光学的方法记录

Article DOI: 10.3791/4052-v • 08:48 min • September 5th, 2012
September 5th, 2012 •
1, 1
1Section of Neurobiology, Center for Learning and Memory, The University of Texas at Austin

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了解脊椎动物的中枢神经系统的功能，需要从许多神经元的录音，因为皮层的功能所产生的，在神经元中的人口水平。在这里，我们描述了一个光学的方法来记录阈上的神经活动与单细胞和单穗分辨率，抖动随机存取扫描。这种方法记录体荧光钙信号与高时间分辨率从多达100个神经元。最大似然的算法deconvolves的基本阈上的神经活动，自体荧光钙信号。这种方法可以可靠地检测具有较高的检测效率和低误报率的尖峰，可以用来研究神经元群

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神经科学杂志，第67期，功能钙成像，时空的活动模式
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