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Neuroscience

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Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
 

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Article DOI: 10.3791/4061-v 10:25 min September 12th, 2012
September 12th, 2012

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Summary

We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.

Tags

Time-lapse Imaging Neuroblast Migration Adult Mouse Forebrain Neural Stem Cells Subventricular Zone (SVZ) Rostral Migratory Stream (RMS) Olfactory Bulb (OB) Astrocytic Processes Blood Vessels Cell Migration Acute Slices Rodent Brain In Vivo Imaging DIC Optics Fluorescent Proteins Viral Labeling
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