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Farvning protokoller for Menneskerettigheder bugspytkirteløer

Article DOI: 10.3791/4068-v • 07:48 min • May 23rd, 2012
May 23rd, 2012 •
1, 1, 1, 1, 1
1Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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Videoen viser fremgangsmåder til karakterisering af humane pancreasøer med hematoxylin og eosin (H & E) og immunhistokemi (IHC). Pancreatiske sektioner fra hoved, krop og hale regioner farves med både H & E og IHC at bestemme ø endokrine sammensætning (insulin, glucagon og pancreatisk polypeptid), cellereplikation (Ki67) og inflammatoriske infiltrater (H & E, CD3). Den uncinate region er lokaliseret ved hjælp IHC for pancreatisk polypeptid.

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Medicin Physiology type 1 diabetes histologi H & E immunhistokemi insulin beta-celler glucagon alfa-celler pancreatisk polypeptid ø pancreas milt organdonor
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