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Neuroscience

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Preparação de fatias agudas zona subventricular for Imaging Cálcio
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Preparação de fatias agudas zona subventricular for Imaging Cálcio

Article DOI: 10.3791/4071
September 19th, 2012 •
1, 1, 1, 1
1Department of Neurosurgery and Cellular & Molecular Physiology, Yale University School of Medicine

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September 19th, 2012

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Um método para carregar subventricular zona (SVZ) de células com corantes de cálcio indicador de actividade de cálcio gravação é descrito. A SVZ pós-natal contém células hermeticamente embalados, incluindo as células progenitoras neurais e neuroblastos. Ao invés de usar o carregamento banho foi injetado o corante por pressão dentro do tecido, permitindo uma melhor difusão de corante.

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