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Il processo di Green Monster per la generazione di ceppi di lieviti trasporto gene delezioni multiple
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Il processo di Green Monster per la generazione di ceppi di lieviti trasporto gene delezioni multiple

Article DOI: 10.3791/4072 • 13:06 min
December 15th, 2012 •
1, 1, 2, 3, 2, 3,4
1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital

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December 15th, 2012

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Il metodo Green Monster consente l'assemblaggio rapido di delezioni multiple contrassegnati con un reporter gene codificante proteina fluorescente verde. Questo metodo si basa sulla guida ceppi di lievito attraverso cicli ripetuti di assortimento sessuale di delezioni e basato sulla fluorescenza arricchimento di cellule che trasportano più eliminazioni.

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