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The Green Monster Werkwijze voor de generatie van giststammen dragen Meerdere gendeleties
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The Green Monster Werkwijze voor de generatie van giststammen dragen Meerdere gendeleties

Article DOI: 10.3791/4072-v • 13:06 min • December 15th, 2012
December 15th, 2012 •
1, 1, 2, 3, 2, 3,4
1Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics, University of Toronto, 4Lunenfeld Research Institute, Mt Sinai Hospital

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De Green Monster methode kan de snelle montage van meerdere deleties gemarkeerd met een reportergen codeert groen fluorescent eiwit. Deze methode is gebaseerd op een giststammen door herhaalde cycli van seksuele assortiment van deleties en fluorescentie gebaseerde verrijking van cellen die meer deleties.

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Microbiologie Genetica Synthetische Biologie Milieu Genomics Genomics Bioengineering Biomedische Technologie Cellular Biology Multi-site genomische engineering genetische interactie groen fluorescerend eiwit GFP flowcytometrie, Gist Green Monster
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