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In vitro-analyse af PDZ-afhængige CFTR Makromolekylære signalering Komplekser
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In vitro-analyse af PDZ-afhængige CFTR Makromolekylære signalering Komplekser

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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Cystisk fibrose transmembran konduktans regulator (CFTR), en epitelcelle chlorid-kanal, er blevet rapporteret at interagere med forskellige proteiner og regulere vigtige cellulære processer, deriblandt CFTR PDZ motiv-medierede interaktioner er blevet godt dokumenteret. Denne protokol beskriver de metoder vi har udviklet til at samle en PDZ-afhængig CFTR makromolekylære signalering kompleks

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Biokemi molekylærbiologi kemi CFTR makromolekylære kompleks protein interaktion PDZ stillads protein epitelcelle cystisk fibrose
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