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Prøvetaking Menneskelig Indigenous Spytt Peptidome Bruke en Lollipop-Like ultrafiltrasjon Probe
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Prøvetaking Menneskelig Indigenous Spytt Peptidome Bruke en Lollipop-Like ultrafiltrasjon Probe: Forenkle og forbedre Peptide Detection for klinisk massespektrometri

Article DOI: 10.3791/4108-v • 08:37 min • August 7th, 2012
August 7th, 2012 •
1, 2,3, 2,3,4
1Sanford-Burnham Medical Research Institute, 2Division of Dermatology, University of California, San Diego, 3VA San Diego Healthcare Center, 4Moores Cancer Center, University of California, San Diego

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Vurderer spytt prøvetaking for fremtidig klinisk anvendelse, ble en lollipop-lignende ultrafiltrasjon (LLUF) sonde fabrikert for å passe i den menneskelige munnhulen. Direkte analyse av ufordøyd spytt ved NanoLC-LTQ massespektrometri demonstrert evne LLUF sonder for å fjerne store proteiner og høye overflod proteiner, og gjør lite tallrike peptider mer synlig.

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Medisin molekylærbiologi genetikk Sampling spytt Peptidome ultrafiltrasjon Massespektrometri
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