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Souris îlot de Langerhans isolement à l'aide d'une combinaison de la collagénase purifiée et protéase neutre

Article DOI: 10.3791/4137-v • 06:59 min • September 7th, 2012
September 7th, 2012 •
1, 2, 2, 1, 1,3,4
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine

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Une description détaillée de la souris îlot isolement est décrit en utilisant la technique de la ponction du pancréas in situ canalaire et la perfusion d'une combinaison de collagénase purifiée et la protéase neutre.

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Biologie cellulaire numéro 67 Islet la collagénase la souris l'insuline la fluorescence
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