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Journal / Biology / Rato Ilhéu de Langerhans isolamento usando uma…
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Rato Ilhéu de Langerhans isolamento usando uma combinação de colagenase purificada e Protease Neutra
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Rato Ilhéu de Langerhans isolamento usando uma combinação de colagenase purificada e Protease Neutra

Article DOI: 10.3791/4137-v • 06:59 min • September 7th, 2012
September 7th, 2012 •
1, 2, 2, 1, 1,3,4
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine

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Uma descrição detalhada de isolamento de ilhotas do rato está descrito por meio da técnica de canulação na pancreático ductal in situ e a perfusão de uma combinação de colagenase purificados e protease neutra.

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Biologia Celular Edição 67 Ilhota colagenase mouse insulina fluorescência
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