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Biology

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小规模的核提取物,基因表达机具功能分析
 
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小规模的核提取物,基因表达机具功能分析

Article DOI: 10.3791/4140-v 08:05 min June 27th, 2012
June 27th, 2012

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Summary

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Click here for the English version.

一个健壮的,小规模的HeLa细胞的核提取物制备的协议。该协议是为需要使用小种群的细胞,如细胞治疗药物或RNAi,有价值的实验。该方法应该适用于各种各样的基因表达分析和其他类型的细胞,包括病人的细胞。

Tags

细胞生物学,64期,遗传学,HeLa细胞核提取物,小规模的提取物,前体mRNA剪接,RNA聚合酶II转录,RNA干扰,加上转录/剪接,
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