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JoVE Journal Neuroscience
An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice
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An Optimized Procedure for Fluorescence-activated Cell Sorting (FACS) Isolation of Autonomic Neural Progenitors from Visceral Organs of Fetal Mice

Article DOI: 10.3791/4188-v • 12:07 min • August 17th, 2012
August 17th, 2012 •
1, 1, 1, 1
1Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine

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Summary
Automatic Translation

An optimized procedure to purify neural crest-derived neuronal progenitors from fetal mouse tissues is described. This method takes advantage of expression from fluorescent reporter alleles to isolate discrete populations by fluorescence-activated cell sorting (FACS). The technique can be applied to isolate neuronal subpopulations throughout development or from adult tissues.

Tags

Fluorescence-activated Cell Sorting FACS Autonomic Neural Progenitors Visceral Organs Fetal Mice Neural Crest Autonomic Ganglia Laser Capture Micro-dissection Fluorescent Reporters Tyrosine Hydroxylase TH Gene Catecholamines Enteric Neuronal Progenitors Migration Fetal Intestine Pelvic Ganglia Neurons EGFP Transgene Construct
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