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Journal / Immunology and Infection / Massaspectrometrische analyse van…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Massaspectrometrische analyse van glycosfingolipiden antigenen
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Massaspectrometrische analyse van glycosfingolipiden antigenen

Article DOI: 10.3791/4224-v • 13:09 min • April 16th, 2013
April 16th, 2013 •
1, 2, 3,4
1Undergraduate Program, Rice University, 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center, 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 4University of Texas Graduate School of Biological Sciences at Houston

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Een specifieke en gevoelige methode om inzicht te krijgen in de expressie profiel van glycosfingolipiden antigenen in het immuunsysteem organen en cellen wordt beschreven. De methode maakt gebruik van de ion trap massaspectrometrie waardoor stapsgewijze versnippering van glycosfingolipiden moleculen voor structurele analyse in vergelijking met chemisch gesynthetiseerde standaard.

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Immunologie Biochemie Moleculaire Biologie Cellular Biology Structurele Biologie Geneeskunde Genetica Proteomics Proteïnen Glycomics Functioneel glycomics glycosfingolipiden GSLs antigenen natural killer T-cellen MALDI-TOF massaspectrometrie LTQ ionenval massa spectrometer massaspectrometrie glycolipiden lipiden
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