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Studere Proteolyse af cyclin B på enkelt celle niveau i hele cellepopulationer
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Studere Proteolyse af cyclin B på enkelt celle niveau i hele cellepopulationer

Article DOI: 10.3791/4239-v • 10:55 min • September 17th, 2012
September 17th, 2012 •
1, 1, 1, 1, 1
1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

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Metafase at anafase overgang udløses gennem anaphase-fremmende kompleks (APC / C)-afhængig ubiquitinering og efterfølgende destruktion af cyclin B. Her har vi oprettet et system, som efter puls-chase mærkning, mulighed for at overvåge cyclin B proteolyse i hele cellepopulationer og letter påvisning af indblanding fra mitotisk checkpoint.

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Genetik Cellular Biology Molecular Biology Proteomics Cyclin B spindel samling checkpointet anaphase-fremme kompleks mitose proteasom-afhængig proteolyse SNAP cellecyklus
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