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JoVE Journal Biology
전체 세포 인구의 단일 셀 수준에서 Cyclin B의 Proteolysis 유학
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전체 세포 인구의 단일 셀 수준에서 Cyclin B의 Proteolysis 유학

Article DOI: 10.3791/4239
September 17th, 2012 •
1, 1, 1, 1, 1
1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

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September 17th, 2012

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전환이 anaphase-추진 단지 (APC / C)에 의존 ubiquitination과 여기 cyclin B.의 후속 파괴를, 우리는, 펄스 - 체이스 라벨에 따라, 시스템을 설립 전체 세포 인구에서 cyclin B의 proteolysis를 모니터링 할 수 있으며를 통해 실행됩니다 anaphase에 Metaphase mitotic 검문소에 의한 간섭의 검색을 용이하게한다.

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