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L'uso di LysoTracker per rilevare la morte cellulare programmata in embrioni e differenziazione delle cellule staminali embrionali
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L'uso di LysoTracker per rilevare la morte cellulare programmata in embrioni e differenziazione delle cellule staminali embrionali

Article DOI: 10.3791/4254-v • 12:44 min • October 11th, 2012
October 11th, 2012 •
1, 1, 1
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

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Vi presentiamo un protocollo semplice per visualizzare le regioni di morte cellulare programmata (PCD) in embrioni di topo e di differenziazione staminali embrionali (ES) colture cellulari utilizzando un colorante chiamato LysoTracker altamente solubile.

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Biologia dello Sviluppo Numero 68 biologia molecolare biologia delle cellule staminali Biologia Cellulare embrione di topo le cellule staminali embrionali lisosomi la morte programmata delle cellule l'imaging sonic hedgehog
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