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Substrat Generation for endonukleaser af CRISPR / Cas Systems
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Substrat Generation for endonukleaser af CRISPR / Cas Systems

Article DOI: 10.3791/4277-v • 11:53 min • September 8th, 2012
September 8th, 2012 •
1, 1, 1, 1, 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology

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CRISPR / Cas-systemer medierer adaptive immunitet i Bacteria og Archaea. Mange Cas proteiner foreslået at fungere som endoribonucleases virker på crRNA forstadier af varierende længde. Her viser vi tre forskellige tilgange til at generere pre-crRNA substrater for den biokemiske analyse af Cas endonuclease aktivitet.

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Molekylær biologi CRISPR / Cas endonuclease, crRNA Cas6
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