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Journal / Biology / Substrat Generation für Endonukleasen der CRISPR…
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Substrat Generation für Endonukleasen der CRISPR / Cas-Systeme
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Substrat Generation für Endonukleasen der CRISPR / Cas-Systeme

Article DOI: 10.3791/4277-v • 11:53 min • September 8th, 2012
September 8th, 2012 •
1, 1, 1, 1, 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology

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CRISPR / Cas-Systeme vermitteln adaptive Immunität in Bakterien und Archaea. Viele Cas Proteine ​​vorgeschlagen, als Endoribonukleasen die auf crRNA Vorläufer von unterschiedlicher Länge handeln. Hier erläutern wir drei verschiedene Ansätze vor, crRNA Substrate für die biochemische Analyse von Cas Endonucleaseaktivität generieren.

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Molekularbiologie CRISPR / Cas Endonuklease, crRNA Cas6
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