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Journal / Biology / Generazione Substrato per endonucleasi di CRISPR…
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Generazione Substrato per endonucleasi di CRISPR / Cas Sistemi
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Generazione Substrato per endonucleasi di CRISPR / Cas Sistemi

Article DOI: 10.3791/4277-v • 11:53 min • September 8th, 2012
September 8th, 2012 •
1, 1, 1, 1, 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology

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CRISPR / Cas sistemi mediata immunità adattativa in Batteri e Archaea. Molte proteine ​​Cas si propongono di agire come agiscono sul endoribonucleases crRNA precursori di varia lunghezza. Qui si illustrano tre diversi approcci per la generazione pre-crRNA substrati per l'analisi biochimica di attività endonucleasi Cas.

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Biologia molecolare Numero 67 CRISPR / Cas endonucleasi, crRNA Cas6
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