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Substrat Generation for endonukleaser av CRISPR / Cas Systems
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Substrat Generation for endonukleaser av CRISPR / Cas Systems

Article DOI: 10.3791/4277-v • 11:53 min • September 8th, 2012
September 8th, 2012 •
1, 1, 1, 1, 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology

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CRISPR / Cas-systemer megle adaptiv immunitet i bakterier og Archaea. Mange Cas proteiner er foreslått å fungere som endoribonucleases virker på crRNA forløpere av varierende lengde. Her har vi illustrere tre ulike tilnærminger for å generere pre-crRNA substrater for den biokjemiske analysen av Cas endonuclease aktivitet.

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Molekylærbiologi CRISPR / Cas endonuclease, crRNA CAS-6
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