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JoVE Journal
Bioengineering

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使用电化学传感器的细菌检测和鉴定
 
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使用电化学传感器的细菌检测和鉴定

Article DOI: 10.3791/4282-v 09:30 min April 23rd, 2013
April 23rd, 2013

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Summary

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Click here for the English version.

我们描述了一种电化学传感器的测定方法,细菌的快速检测和识别。的测定法涉及官能化的传感器阵列与DNA的核糖体RNA(rRNA基因)的种属特异性序列的寡核苷酸捕捉探针。目标rRNA基因的捕获探针和辣根过氧化物酶相连的DNA寡核苷酸检测探针的夹心杂交产生可测量的安培电流。

Tags

生物工程,第74期,微生物学,遗传学,分子生物学,细胞生物学,生物化学,生物医学工程,医学,免疫学,细菌,电化学传感器,核糖体RNA,rRNA基因,16S RNA,DNA,探针,检测
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