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Journal / Bioengineering / Bakteriel Detection og identifikation Brug…
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Bioengineering

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JoVE Journal Bioengineering
Bakteriel Detection og identifikation Brug elektrokemiske sensorer
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Bakteriel Detection og identifikation Brug elektrokemiske sensorer

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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Vi beskriver en elektrokemisk sensor analysemetode for hurtig påvisning af bakterier og identifikation. Assayet involverer et sensorarray funktionaliseret med DNA oligonucleotid capture prober til ribosomale RNA (rRNA) artsspecifikke sekvenser. Sandwich hybridisering af mål-rRNA med indfangningsproben og en peberrodsperoxidase-bundet DNA-oligonukleotid detektorprobe frembringer en målelig amperometrisk strøm.

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Bioteknik mikrobiologi genetik molekylærbiologi cellebiologi biokemi Biomedical Engineering Medicine Immunologi Bakterier elektrokemisk sensor ribosomale RNA rRNA 16S RNA DNA probe assay
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