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Journal / Bioengineering / Bacteriana Detecção e Identificação Utilizando…
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JoVE Journal Bioengineering
Bacteriana Detecção e Identificação Utilizando sensores eletroquímicos
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Bacteriana Detecção e Identificação Utilizando sensores eletroquímicos

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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Descreve-se um método de ensaio de sensor electroquímico para a detecção bacteriana rápida e identificação. O ensaio envolve uma matriz de sensores funcionalizada com as sondas de DNA para o RNA de captura de oligonucleótidos (rRNA) sequências específicas das espécies ribossomal. Sandwich de rRNA alvo de hibridação com a sonda de captura e uma sonda detectora de peroxidase de rábano ligada ao oligonucleótido de DNA produz uma corrente mensurável amperométrica.

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Bioengenharia Microbiologia Genética Biologia Molecular Biologia Celular Bioquímica Engenharia Biomédica Medicina Imunologia bactérias sensor eletroquímico RNA ribossômico rRNA 16S RNA DNA a sonda o ensaio de
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