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La valutazione delle funzioni mitocondriali e la vitalità cellulare in cellule renali proteina chinasi C overexpressing isoenzimi

Article DOI: 10.3791/4301
January 7th, 2013 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences

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January 7th, 2013

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Gli effetti di attivazione di proteina chinasi C (PKC) isoenzimi sulle funzioni mitocondriali associate respirazione e fosforilazione ossidativa e sulla vitalità cellulare sono descritti. L'approccio si adatta tecnica adenovirale per sovraesprimere selettivamente isoenzimi PKC in coltura primaria e una varietà di saggi per determinare le funzioni mitocondriali e lo stato energetico della cellula.

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