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Beoordeling van Mitochondriale functies en levensvatbaarheid van de cellen in de nier overexpressie Proteïne kinase C-isozymen
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Beoordeling van Mitochondriale functies en levensvatbaarheid van de cellen in de nier overexpressie Proteïne kinase C-isozymen

Article DOI: 10.3791/4301-v • 15:43 min • January 7th, 2013
January 7th, 2013 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences

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De effecten van activering van eiwit kinase C (PKC) isozymen op mitochondriële functies geassocieerd met ademhaling en oxidatieve fosforylering en cellevensvatbaarheid beschreven. De aanpak past adenovirale techniek om selectief PKC overexpressie isozymen in primaire celkweek en verschillende assays voor mitochondriële functies en energie status van de cel te bepalen.

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Cellular Biology Biochemie Moleculaire Biologie Genetica farmacologie fysiologie geneeskunde Protein Mitochondriale dysfunctie mitochondria proteïne kinase C renale proximale tubulaire cellen reactive oxygen species zuurstofverbruik elektronen transportketen ademhaling complexen ATP adenovirus primaire kweek ischemie cellen flowcytometrie
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