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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Article DOI: 10.3791/4322-v • 14:48 min • December 26th, 2012
December 26th, 2012 •
1,2, 1,2, 3, 2, 1,2, 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

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Summary
Automatic Translation

We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

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Flow Cytometric Isolation Primary Murine Type II Alveolar Epithelial Cells Functional Studies Molecular Studies Immune Regulation Cytokine Production Antigen-presenting Cells Infection T-cell Mediated Autoimmunity Clinical Contexts Airway Hyper-reactivity Infections Cell Lines Large Numbers Of Cells Complex Processes Autoimmune Inflammation
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