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Generation of Human induzierten pluripotenten Stammzellen aus dem peripheren Blut mit dem STEMCCA Lentivirusvektor
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Generation of Human induzierten pluripotenten Stammzellen aus dem peripheren Blut mit dem STEMCCA Lentivirusvektor

Article DOI: 10.3791/4327-v • 12:03 min • October 31st, 2012
October 31st, 2012 •
1, 1, 2, 3, 1, 1, 1, 3, 1, 3, 1, 1
1Center for Regenerative Medicine (CReM), Boston University School of Medicine, 2Department of Hematology, Children's Hospital of Philadelphia, 3Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia

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Hier zeigen wir, ein einfaches und effektives Protokoll für die Herstellung von menschlichen IPSCs 3-4 ml peripheres Blut unter Verwendung eines einzigen lentiviralen Vektors Umprogrammierung. Reprogrammierung von leicht verfügbaren Blutkörperchen verspricht die Nutzung von iPS-Technologie, indem sie Zugang zu einem breiteren Forschungsgemeinschaft beschleunigen.

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Stem Cell Biology Ausgabe 68 induzierte pluripotente Stammzellen (iPS) periphere mononukleäre Blutzellen (PBMCs) Neuprogrammierung Single verbrauchsteuerpflichtiger lentiviralen Vektor STEMCCA
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