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Handy-Tracking Mit Photoconvertible Proteine ​​während der Zebrafisch-Entwicklung
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JoVE Journal Biology
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
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Handy-Tracking Mit Photoconvertible Proteine ​​während der Zebrafisch-Entwicklung

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11,258 Views

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07:19 min

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September 28, 2012

DOI:

10.3791/4350-v

DOI:
10.3791/4350-v

•

07:19 min
September 28, 2012

•

3 Views
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1Max Delbrück Center for Molecular Medicine

Summary

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Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.

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Cite this Article

Lombardo, V. A., Sporbert, A., Abdelilah-Seyfried, S. Cell Tracking Using Photoconvertible Proteins During Zebrafish Development. J. Vis. Exp. (67), e4350, doi:10.3791/4350 (2012).

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