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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Article DOI: 10.3791/4372-v • 14:53 min • November 24th, 2012
November 24th, 2012 •
1, 1, 2
1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

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Summary
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The method described isolation and characterization of human Dental Pulp Stem Cells (hDPSCs) by using either enzymatic dissociation of pulp (DPSC-ED) or direct outgrowth of stem cells from pulp tissue explants (DPSC-OG). Then followed by in vitro comparative differentiation of both types of hDPSCs into odontoblasts.

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Isolation Characterization Comparative Differentiation Human Dental Pulp Stem Cells Permanent Teeth Two Different Methods Wisdom Teeth Routine Orthodontic Treatments HDPSCs Proliferation Potential Multi-lineage Differentiation Capacity Autologous Transplantation Tissue Engineering Regenerative Medicine Mesenchymal Stem Cells MSC Features Immunomodulatory Effect Allograft Transplantation Isolation Conditions Surface Markers Differentiation Capacity Pulp Tissue Impacted Third Molar Teeth Enzymatic Dissociation Of Pulp Tissue (DPSC-ED) Outgrowth From Tissue Explants (DPSC-OG) Dental Diamond Disk
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