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RNA-seq Analyse van transcriptomen in trombine-behandelde en Control Human Pulmonary microvasculaire endotheelcellen
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RNA-seq Analyse van transcriptomen in trombine-behandelde en Control Human Pulmonary microvasculaire endotheelcellen

Article DOI: 10.3791/4393-v • 18:30 min • February 13th, 2013
February 13th, 2013 •
1, 1, 1, 1, 1, 1, 1
1Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City

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Dit protocol geeft een volledige en gedetailleerde procedure voor RNA-seq, een krachtige volgende generatie DNA sequencing technologie, toepassing profiel transcriptomen in menselijke long microvasculaire endotheliale cellen met of zonder behandeling trombine. Dit protocol is generaliseerbaar verschillende cellen of weefsels die door verschillende reagentia of ziekten.

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Genetics moleculaire biologie immunologie Medicine Genomics Proteins RNA-seq Next Generation DNA Sequencing Transcriptome Transcription trombine endotheelcellen high-throughput DNA genomisch DNA RT-PCR PCR
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