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从基因组DNA选择性捕获的5 - 羟甲基
 
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从基因组DNA选择性捕获的5 - 羟甲基

Article DOI: 10.3791/4441-v 06:26 min October 5th, 2012
October 5th, 2012

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

描述的是一个两步骤的标记过程中使用的β-葡糖基转移酶(β-GT)来传输的叠氮化物5-HMC-葡萄糖,然后通过点击化学生物素链接器为容易和密度独立富集转移。这种高效的和具体的标记方法使富集5 - HMC具有极低的背景和高通量表观映射的通过新一代测序。

Tags

68,化学,生物物理,化学品标签,5 - 羟甲基高通量测序的基因组DNA,遗传学,
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