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Undersøgelse af DNA beskadigelse checkpoint'et ved hjælp
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Undersøgelse af DNA beskadigelse checkpoint'et ved hjælp

Article DOI: 10.3791/4449-v • 10:55 min • November 5th, 2012
November 5th, 2012 •
*1, *1, 1, 1, 1
1Department of Biology, University of North Carolina at Charlotte
* These authors contributed equally

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Xenopus æggeekstrakt er et nyttigt model-system til at undersøge DNA beskadigelse checkpoint'et. Denne protokol er til fremstilling af Xenopus æg ekstrakter og DNA beskadigelse checkpoint'et inducerende reagenser. Disse teknikker kan tilpasses til en række DNA-ødelæggende fremgangsmåder i undersøgelsen af ​​DNA beskadigelse checkpoint'et signalering.

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Genetik Molecular Biology Cellular Biology Developmental Biology DNA-skader kontrolpost, Chk1 phosphorylering ATR AT70 MMS UV immunoblotting
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