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Journal / Neuroscience / Analys DNA-skador i hippocampus neuroner Använda…
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Neuroscience

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JoVE Journal Neuroscience
Analys DNA-skador i hippocampus neuroner Använda Comet Assay
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Analys DNA-skador i hippocampus neuroner Använda Comet Assay

Article DOI: 10.3791/50049-v • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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Kometen analysen är ett effektivt sätt att upptäcka enkel-och dubbel-strängbrott, inklusive alkali-labila platser och DNA-DNA/DNA-protein tvärbindningar på DNA i alla celler, inklusive hippocampus neuroner. Metoden utnyttjar den differentiella migreringen av DNA i ett elektriskt fält på grund av skillnader i mängden av DNA-skada.

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Neurovetenskap genetik Cellulär biologi medicin Cancer Biology anatomi fysiologi DNA DNA-skador dubbelsträng paus enkelsträngad paus reparation neuroner komet analys cellodling
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