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Journal / Bioengineering / Procedimiento para descelularización de corazón…
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JoVE Journal Bioengineering
Procedimiento para descelularización de corazón porcino por perfusión coronaria retrógrada
Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA)
Journal (Bioengineering)
Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA)
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
Journal (Bioengineering)
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
 
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Procedimiento para descelularización de corazón porcino por perfusión coronaria retrógrada

Article DOI: 10.3791/50059-v • 11:30 min • December 6th, 2012
December 6th, 2012 •
1,2, 1,3, 1,2,3,4
1McGowan Institute for Regenerative Medicine, 2Department of Bioengineering, University of Pittsburgh, 3Department of Cardiothoracic Surgery, Children's Hospital of Pittsburgh of UPMC, 4Department of Surgery, University of Pittsburgh

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Un método para eliminar rápida y completamente los componentes celulares de un corazón porcino intacta a través de perfusión retrógrada se describe. Este método rinde un sitio específico cardíaco extracelular andamio matriz que tiene el potencial para su uso en diversas aplicaciones clínicas.

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Bioingeniería Número 70 Tissue Engineering Ingeniería Biomédica Biología Celular Medicina Cardiología matriz extracelular descelularización modelo animal porcino cardíacas tejido del corazón
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