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Visualisering av endoplasmatiska nätverket Lokaliserade mRNA i däggdjursceller
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Visualisering av endoplasmatiska nätverket Lokaliserade mRNA i däggdjursceller

Article DOI: 10.3791/50066-v • 10:24 min • December 17th, 2012
December 17th, 2012 •
1, 1
1Department of Biochemistry, University of Toronto

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Här beskriver vi en metod för att visualisera endoplasmatiska retiklet-associerade mRNA i däggdjursceller vävnadsodlingsceller. Denna teknik inbegriper selektiv permeabilisering av plasmamembranet med digitonin att avlägsna cytoplasmiska innehåll följt av fluorescerande

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Cellulär Biology biokemi genetik molekylärbiologi Genomics mRNA lokalisering RNA digitonin extraktion cell-fraktionering endoplasmatiskt retikulum sekretion mikroskopi bildbehandling fluorescerande FISH cellbiologi
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