Biology
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Gene Trapping Mit Gal4 in Zebrafisch
Chapters
Summary September 29th, 2013
Please note that all translations are automatically generated.
Click here for the English version.
Dieses Protokoll beschreibt die Methode des Gene-Trap-Insertionsmutagenese mit Gal4-VP16 als primäre Reporter und GFP / RFP als Sekundär Reportern im Zebrafisch. Etwa einer von zehn hoch-exprimierenden F0 Fischertrag Genfallen Nachkommen Co-GFP und RFP. Das Screening-Verfahren kann leicht skaliert werden, um auf die Größe des Labors Durchführung der Insertionsmutagenese Bildschirm anzupassen.
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