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Journal / Biology / Zebra balığı Gal4 kullanma Gene Yakalama
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Zebra balığı Gal4 kullanma Gene Yakalama
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Zebra balığı Gal4 kullanma Gene Yakalama

Article DOI: 10.3791/50113-v • 13:34 min • September 29th, 2013
September 29th, 2013 •
1, 1
1Department of Biology, Temple University

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Bu protokol Zebrafish ikincil muhabir olarak birincil ve GFP raportör / RFP olarak Gal4-VP16 kullanılarak gen trap girmeyle mutagenez yöntemini anlatır. Yaklaşık bir yüksek ifade F0 balık verim gen trap döl GFP ve RFP co-ifade on. Tarama prosedürü kolayca sokma mutajenez ekranını performans laboratuvar boyutuna uyum ölçekli olabilir.

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Gelişimsel Biyoloji Sayı 79 Zebra balığı Mutagenesis Genetik genetik (hayvan ve bitki) Gal4 transposon gen tuzak sokma mutajenez
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