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The MultiBac Protein Complex Production Platform at the EMBL
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The MultiBac Protein Complex Production Platform at the EMBL

Article DOI: 10.3791/50159-v • 13:51 min • July 11th, 2013
July 11th, 2013 •
1, 1, 1, 1, 1, 1
1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322

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Summary
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Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.

Tags

MultiBac Protein Complex EMBL Proteomics Eukaryotic Proteomes Biological Activity Assembly Lines Cell Functions Protein Complexes Recombinant Production Baculovirus Expression Vector System (BEVS) Eukaryotic Proteins Post-translational Processing Insect Cell Cultures Gene Of Interest MultiBac BEVS Subunits Expression Experiment
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