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JoVE Journal Bioengineering
Evaluatie van polymere Gene Delivery Nanodeeltjes door Nanodeeltje Tracking Analyse en High-throughput Flow cytometrie
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Evaluatie van polymere Gene Delivery Nanodeeltjes door Nanodeeltje Tracking Analyse en High-throughput Flow cytometrie

Article DOI: 10.3791/50176-v • 08:51 min • March 1st, 2013
March 1st, 2013 •
1,2, 1,2, 1,2,3,4
1Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of Medicine

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Een protocol voor de nanodeeltjes tracking analyse (NTA) en high-throughput flowcytometrie om polymere genafgifte nanodeeltjes evalueren beschreven. NTA wordt gebruikt om de nanodeeltjes deeltjesgrootteverdeling en het plasmide per deeltjesverdeling karakteriseren. High-throughput flowcytometrie in staat stelt kwantitatieve transfectie efficiëntie evaluatie voor een bibliotheek van gen delivery biomaterialen.

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Biomedische Technologie Bioengineering Tissue Engineering Cellulaire Biologie Geneeskunde Genetica biocompatibele materialen Biopolymeren Drug Delivery Systems Nanotechnologie biotechnologie (algemeen) Therapeutics Nanodeeltje poly (beta-amino ester) high-throughput transfectie nanodeeltjes tracking analyse biomateriaal genaflevering flowcytometrie
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