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JoVE Journal Neuroscience
Optogenetic Aktivering af zebrafisk somatosensoriske Neuroner med Chef-tdTomato
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Optogenetic Aktivering af zebrafisk somatosensoriske Neuroner med Chef-tdTomato

Article DOI: 10.3791/50184-v • 07:25 min • January 31st, 2013
January 31st, 2013 •
1, 1
1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles

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Optogenetic teknikker har gjort det muligt at studere bidrag fra bestemte neuroner til adfærd. Vi beskriver en metode i larvernes zebrafisk til aktivering enkelt somatosensoriske neuroner udtrykker et channelrhodopsin variant (kok) med en diode-pumpet solid state (DPSS) laser og registrere fremkaldte adfærd med en high-speed video kamera.

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Neuroscience Developmental Biology Molecular Biology Cellular Biology Biochemistry Bioengineering anatomi fysiologi zebrafisk Behavior Animal Touch optogenetics channelrhodopsin kok sensorisk neuron Rohon-Beard, Neuroner mikroinjektion konfokal mikroskopi high speed video dyremodel
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