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JoVE Journal Neuroscience
Optogenetic Aktivering av zebrafisk somatosensoriska nervceller med kock-tdTomato
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Optogenetic Aktivering av zebrafisk somatosensoriska nervceller med kock-tdTomato

Article DOI: 10.3791/50184-v • 07:25 min • January 31st, 2013
January 31st, 2013 •
1, 1
1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles

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Optogenetic tekniker har gjort det möjligt att studera bidrag specifika nervceller till beteende. Vi beskriver en metod i larver zebrafisk för att aktivera enskilda somatosensoriska neuroner som uttrycker en channelrhodopsin variant (Chef) med en diod-pumpad solid state (DPSS) laser och registrering av framkallade beteenden med en snabb videokamera.

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Neurovetenskap utvecklingsbiologi molekylärbiologi Cellulär biologi biokemi bioteknik anatomi fysiologi zebrafisk beteende djurs Touch optogenetics channelrhodopsin kock sensoriska neuron Rohon-Beard, Neuroner mikroinjektion konfokalmikroskopi hög hastighet video djurmodell
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